Substance 3d modeler beta9/12/2023 ![]() ![]() ![]() This protocol builds upon a large body of previous work for generating β-like cells. This differentiation methodology takes ~34 d to generate functional SC-β cells, plus an additional 1–2 weeks for initial stem cell expansion and final cell assessment. The resulting SC-β cells and other endocrine cell types can then be aggregated into islet-like clusters for analysis and transplantation. Endocrine induction and subsequent SC-β-cell specification is achieved with a cocktail consisting of the cytoskeletal depolymerizing compound latrunculin A combined with XXI, T3, ALK5 inhibitor II, SANT1 and retinoic acid. This protocol first induces definitive endoderm by treatment with Activin A and CHIR99021, then generates PDX1+/NKX6-1+ pancreatic progenitors through the timed application of keratinocyte growth factor, SANT1, TPPB, LDN193189 and retinoic acid. We detail a six-stage planar differentiation methodology for generating human pluripotent stem cell–derived pancreatic β cells (SC-β cells) that secrete high amounts of insulin in response to glucose stimulation. ![]()
0 Comments
Leave a Reply.AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |